![]() With the ESI source, it is attached to a Waters I-Class Plus UPLC for reversed phase and HILIC separations. The QTOF can be fitted with either a DESI source for MS imaging or ESI source for LC-MS experiments. By switching to an ESI source and attaching the I-Class Plus UPLC, this instrument becomes a step up from the Xevo G2-XS owing to its capacity for higher resolution chromatography, higher resolution mass spectra, and ion mobility spectrometry. DESI-imaging is the primary application of this instrument (see MS Imaging page for more details – coming soon). This instrument is available for trained users in collaboration with facility staff. Citation for publicationĪccurate mass measurements/high resolution mass spectra (HRMS) were obtained from the Columbia University Chemistry Department MassSpectrometry Facility on a Waters XEVO G2-XS QToF mass spectrometer equipped with a UPC 2 SFC inlet and a LockSpray source with one of three probes: electrospray ionization (ESI) probe, atmospheric pressure chemical ionization (APCI) probe, or atmospheric pressure solids analysis probe (ASAP). The LC and MS systems are run using MassLynx 4.2, which includes QuanLynx for processing quantitation data and Ma圎nt for intact protein spectra deconvolution. MS/MS, MS E, and TOF-MRM experiments are possible on this instrument. The LockSpray reference mass provides improved mass accuracy and StepWave ion optics provide improved sensitivity. The ion source includes interchangeable ESI and APCI probes as well as an Atmospheric pressure Solids Analysis Probe (ASAP). The QTOF includes a resolving quadrupole, collision cell, and a TOF with a resolving power >40,000 FWHM. Both inlets include a photodiode array detector (PDA) for monitoring UV-vis absorption. A Waters UPC 2 supercritical fluid chromatography system can also be attached for separation of low-to-medium polarity compounds. The QTOF is attached to a Waters H-Class Plus UPLC for reversed phase and HILIC separations. It is also used for quantitation and other experiments designed in collaboration with facility staff. Accurate mass analyses (HRMS) for small molecules are performed on this instrument. This instrument is available for self-service for trained users or by sample submission to be run by facility staff.
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